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Image Search Results
Journal: Scientific Reports
Article Title: Reconstitution of human pyroptotic cell death in Saccharomyces cerevisiae
doi: 10.1038/s41598-023-29464-5
Figure Lengend Snippet: QVD enhanced the proliferation of yeast expressing caspases plus gasdermins, but tolerated concentrations of disulfiram did not. Yeast bearing empty vectors lacking cell death genes ( a ) or plasmids encoding caspase-4 plus GSDMD ( b ), caspase-3 plus GSDME ( c ) or RIPK1, a RHIM mutant of RIPK3 and MLKL (all human) ( d ) were grown in inducing or repressing liquid media containing 1% DMSO and either no drug (white columns) or 1, 2, 4, 8, 16, 32 or 64 µM of disulfiram, QVD or TC13172. The ratio of maximal growth rates in inducing versus repressing media were calculated. Data present mean ± SEM of 3–4 independent assays. Responses to each treatment were compared to untreated samples using one way ANOVAs with Sidak corrections. Statistically significant effects ( P < 0.05) are denoted by asterisks above the columns. A test for trend was performed to identify significant drug responses (either positive or negative). R 2 denotes the fraction of the total variance accounted for by the trend. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Article Snippet: Antibodies used for immunoblotting in this study were: rabbit anti-GSDMD antibody EPR19829 (Abcam, Cambridge, UK), rabbit anti-DFNA5/GSDME antibody EPR19859 (Abcam,), rabbit anti-γHexokinase #4959–9988 (Bio-Rad, Hercules, CA, USA), mouse anti-FLAG T0003 (Affinity Biosciences, Cincinnati, OH, USA), rabbit anti-caspase-1 (A-19) (Santa Cruz Biotechnology, Dallas, TX, USA), rat anti-caspase-2 #MAB3507 (Merck, Darmstadt, Germany),
Techniques: Expressing, Mutagenesis